mcherry tomm20 n 10 (Addgene inc)
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Addgene inc
mcherry tomm20 n 10
Mcherry Tomm20 N 10, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mcherry+tomm20+n/pGEX-KG-D4H*-mCherry+(Plasmid+%23134604)/pm41933731-193-13-14
Average 93 stars, based on 8 article reviews
Mcherry Tomm20 N 10, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mcherry+tomm20+n/pGEX-KG-D4H*-mCherry+(Plasmid+%23134604)/pm41933731-193-13-14
Average 93 stars, based on 8 article reviews
mcherry tomm20 n 10 - by Bioz Stars,
2026-09
93/100 stars
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Construct:Article Title: Incorporating a Polyethyleneglycol Linker to Enhance the Hydrophilicity of Mitochondria-Targeted Triphenylphosphonium Constructs. Article Snippet: The extraction was diluted with 750 μL of HPLC buffer A and filtered through a 0.20 μm 96-well polyethylene filter plate (ThermoFisher Scientific) before analyzing RP-HPLC by the same condition as mitochondrial uptake. .. Plasmids and constructs: Article Title: p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi Article Snippet: BioID-PAQR11 construct was modified from PAQR11-pEGFP-C3 by replacing the EGFP coding sequence with BioID sequence from myc-BioID2-MCS (Addgene plasmid #74223). .. PAQR11 3′UTR luciferase reporters were constructed as previously described ( ). Plasmid Preparation:Article Title: Incorporating a Polyethyleneglycol Linker to Enhance the Hydrophilicity of Mitochondria-Targeted Triphenylphosphonium Constructs. Article Snippet: The extraction was diluted with 750 μL of HPLC buffer A and filtered through a 0.20 μm 96-well polyethylene filter plate (ThermoFisher Scientific) before analyzing RP-HPLC by the same condition as mitochondrial uptake. .. Plasmids and constructs: Article Title: In situ cryo-ET visualization of mitochondrial depolarization and mitophagic engulfment Article Snippet: Detailed protocols may be found here: dx.doi.org/10.17504/protocols.io.81wgbxq2qlpk/v1 ; dx.doi.org/10.17504/protocols.io.yxmvm3z5bl3p/v1 For live time lapse imaging, U2OS cells were plated on glass-bottom 35 mm dishes (Mattek, P35GC-1.5-14-C) 24 h prior to transient plasmid transfection, and 48 h prior to imaging. .. Plasmid transfection occurred in Opti-MEMTM I Reduced Serum Medium (Thermo Fisher Scientific, REF: 31985-070) with Lipofectamine 2000 reagent (Thermo Fisher Scientific, REF:11668030). Article Title: Identification of specific lipid-protein interactions in dividing cells using lipid-trap mass spectrometry Article Snippet: .. pCW57-GFP-2A-MCS was a gift from Adam Karpf (Addgene plasmid # 71783), Lact-C2-GFP was a gift from Sergio Grinstein (Addgene plasmid # 22852), Article Title: p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi Article Snippet: BioID-PAQR11 construct was modified from PAQR11-pEGFP-C3 by replacing the EGFP coding sequence with BioID sequence from myc-BioID2-MCS (Addgene plasmid #74223). .. PAQR11 3′UTR luciferase reporters were constructed as previously described ( ). Article Title: A genome-wide CRISPR-Cas9 knockout screen reveals FSP1 as warfarin-resistant vitamin K reductase Article Snippet: Human Brunello CRISPR knockout 20 pooled library was a gift from David Root and John Doench (Addgene #73178). .. The fluorescent 21 protein-tagged marker of the endoplasmic reticulum (ER) (mCherry-Sec61-N-18), mitochondrial 22 22 ( Article Title: In situ cryo-ET visualization of mitochondrial depolarization and mitophagic engulfment Article Snippet: For live time-lapse imaging, U2OS cells were plated on glass-bottom 35 mm dishes (Mattek, P35GC-1.5-14-C) 24 h prior to transient plasmid transfection, and 48 h prior to imaging. .. Plasmid transfection occurred in Opti-MEMTM I Reduced Serum Medium (Thermo Fisher Scientific, REF: 31985-070) with Lipofectamine 2000 reagent (Thermo Fisher Scientific, REF:11668030). Amplification:Article Title: Incorporating a Polyethyleneglycol Linker to Enhance the Hydrophilicity of Mitochondria-Targeted Triphenylphosphonium Constructs. Article Snippet: The extraction was diluted with 750 μL of HPLC buffer A and filtered through a 0.20 μm 96-well polyethylene filter plate (ThermoFisher Scientific) before analyzing RP-HPLC by the same condition as mitochondrial uptake. .. Plasmids and constructs: Polymerase Chain Reaction:Article Title: Incorporating a Polyethyleneglycol Linker to Enhance the Hydrophilicity of Mitochondria-Targeted Triphenylphosphonium Constructs. Article Snippet: The extraction was diluted with 750 μL of HPLC buffer A and filtered through a 0.20 μm 96-well polyethylene filter plate (ThermoFisher Scientific) before analyzing RP-HPLC by the same condition as mitochondrial uptake. .. Plasmids and constructs: Transfection:Article Title: In situ cryo-ET visualization of mitochondrial depolarization and mitophagic engulfment Article Snippet: Detailed protocols may be found here: dx.doi.org/10.17504/protocols.io.81wgbxq2qlpk/v1 ; dx.doi.org/10.17504/protocols.io.yxmvm3z5bl3p/v1 For live time lapse imaging, U2OS cells were plated on glass-bottom 35 mm dishes (Mattek, P35GC-1.5-14-C) 24 h prior to transient plasmid transfection, and 48 h prior to imaging. .. Plasmid transfection occurred in Opti-MEMTM I Reduced Serum Medium (Thermo Fisher Scientific, REF: 31985-070) with Lipofectamine 2000 reagent (Thermo Fisher Scientific, REF:11668030). Article Title: In situ cryo-ET visualization of mitochondrial depolarization and mitophagic engulfment Article Snippet: For live time-lapse imaging, U2OS cells were plated on glass-bottom 35 mm dishes (Mattek, P35GC-1.5-14-C) 24 h prior to transient plasmid transfection, and 48 h prior to imaging. .. Plasmid transfection occurred in Opti-MEMTM I Reduced Serum Medium (Thermo Fisher Scientific, REF: 31985-070) with Lipofectamine 2000 reagent (Thermo Fisher Scientific, REF:11668030). Luciferase:Article Title: p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi Article Snippet: BioID-PAQR11 construct was modified from PAQR11-pEGFP-C3 by replacing the EGFP coding sequence with BioID sequence from myc-BioID2-MCS (Addgene plasmid #74223). .. PAQR11 3′UTR luciferase reporters were constructed as previously described ( ). Marker:Article Title: A genome-wide CRISPR-Cas9 knockout screen reveals FSP1 as warfarin-resistant vitamin K reductase Article Snippet: Human Brunello CRISPR knockout 20 pooled library was a gift from David Root and John Doench (Addgene #73178). .. The fluorescent 21 protein-tagged marker of the endoplasmic reticulum (ER) (mCherry-Sec61-N-18), mitochondrial 22 22 ( |
